Study of metal-containing proteins in the roots of Eisholtzia splendens using LA-ICP-MS and LC-tandem mass spectrometry
نویسندگان
چکیده
In the present study. the metal-containing proteins in the roots ofCu-tole rant plant ElsllOltzia splendens were investigated. The proteins from the plant roots were extracted and separated by two-dimensional gel electrophoresis (2D GE). Las er ablation inductively coupl ed plasma mass spectrometry (LA-ICP-MS) was used to screen metal-containing proteins on the dried 2D gels. Images of Cu. Zn. Na. Mg. K. Ca. Mn , Fe. P and S. and also of Ag. were obtained by scanning the gel (line by line) using the LA-ICP-MS techniques deve loped in our laboratory. Cu-. Zn-and Mg-containing proteins were found in the gel sections ofinterest via LA-ICP-MS imaging. These protein spots were then in-gel digested with trypsin and analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) for identification. The mechanisms involved in Cu tolerance and accumulation were also discussed.
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